The US company, which opened its first UK franchise in Brighton in 1996 and now has 1,500 across the British Isles, plans to double the number of stores across Bedfordshire, Hertfordshire and Cambridgeshire in the next five years, creating 350 jobs for local people.
There are currently 36 Subway stores across the counties, and plans are in place to increase this number to 72.
The plans include new stores for St Neots and Cambridge, and the company is inviting potential franchisees to apply.
Stacey Moore, Subway brand development agent for the region, said: “We are looking for hard-working, self-motivated and passionate individuals.